I've collected about a dozen spore prints over the past year and I'm wondering about storage. Room temp in envelopes? Refrigerator? Freezer? I've heard conflicting things about whether freezing damages spores. What's the actual best practice for long-term storage?
Reply #1 · ▲ 356 upvotes
For anything less than 2 years: sealed in foil or paper, dark, room temperature or refrigerator. Refrigerator extends viability somewhat. The enemy is moisture, not temperature (at reasonable ranges). For 5+ years: freezer in an airtight container with desiccant, wrapped in foil first.
Reply #2 · ▲ 289 upvotes
The moisture point is crucial and often missed. Condensation when removing cold-stored prints is a spore killer. Always let cold containers come to room temperature before opening them. This one step prevents the majority of long-term storage failures.
Reply #3 · ▲ 312 upvotes
I've successfully germinated prints that were refrigerated for 8 years. The rate drops and you may need more inoculation volume, but aged prints can still work. Frozen prints I've had success with at 4 years. Beyond that, convert to liquid culture for safety.
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